2ZZK
Crystal structure of tRNA wybutosine synthesizing enzyme TYW4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.834, 90.139, 251.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.389 - 2.706 |
| R-factor | 0.216 |
| Rwork | 0.210 |
| R-free | 0.27350 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.826 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 48148 | |
| <I/σ(I)> | 30.7 | 4 |
| Completeness [%] | 96.6 | 76.3 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 200 mM ammonium citrate (pH 7) and 20 % (w/v) PEG3,350, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
