2ZZJ
Crystal structure of endo-beta-1,4-glucuronan lyase from fungus Trichoderma reesei
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000, 0.97877, 0.97926, 0.96403 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.287, 62.301, 129.121 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.940 - 1.800 |
R-factor | 0.18563 |
Rwork | 0.184 |
R-free | 0.22346 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.402 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 27647 | |
<I/σ(I)> | 30.8 | 4 |
Completeness [%] | 98.5 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG3350, 5.0mM CaCl2, 0.2M ammonium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |