2ZZG
Crystal structure of alanyl-tRNA synthetase in complex with 5''-O-(N-(L-alanyl)-sulfamyoxyl) adenine without oligomerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.874, 89.205, 94.905 |
| Unit cell angles | 117.31, 90.40, 107.36 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.20309 |
| Rwork | 0.199 |
| R-free | 0.27211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1riq 1v7o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0039) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.085 | 0.483 |
| Number of reflections | 34772 | |
| <I/σ(I)> | 11.1 | 2.8 |
| Completeness [%] | 96.7 | 96.8 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | PEG6000, MES, succinic acid, glucose, pH 5.6, VAPOR DIFFUSION, temperature 293K |
