2ZZF

Crystal structure of alanyl-tRNA synthetase without oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	164.258, 98.840, 72.927
Unit cell angles	90.00, 108.64, 90.00

Refinement procedure

Resolution	30.150 - 2.700
R-factor	0.208
Rwork	0.206
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1riq 1v7o
RMSD bond length	0.012
RMSD bond angle	1.454
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.050	0.037	0.286
Number of reflections	29921
<I/σ(I)>	44.059
Completeness [%]	98.5	98	88.1
Redundancy	5.3	5.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	PEG6000, MES, succinic acid, glucose, pH 5.6, vapor diffusion, temperature 293K