2ZZ9
Structure of aquaporin-4 S180D mutant at 2.8 A resolution by electron crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ELECTRON MICROSCOPE |
| Source details | OTHER |
| Temperature [K] | 4.2 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | Tietz |
| Wavelength(s) | 2.8 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 69.000, 69.000, 160.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d57 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.524 |
| Data reduction software | MRC |
| Data scaling software | MRC |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.890 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.197 | |
| Number of reflections | 8750 | |
| Completeness [%] | 87.0 | 80.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6 | 293 | 10mM MES (pH 6.0), 75mM NaCl, 50mM MgCl2, 2mM DTT, 1% glycerol, MICRODIALYSIS, temperature 293K |
