2ZZ8
Crystal structure of LipL32, the most abundant surface protein of pathogenic leptospira spp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 125.895, 125.895, 95.991 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.880 - 2.010 |
R-factor | 0.1831 |
Rwork | 0.181 |
R-free | 0.21630 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.392 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 | 0.720 |
Number of reflections | 59453 | |
<I/σ(I)> | 41.9 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.4 | 20.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |