2ZYK
Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin
Replaces: 2DFZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.399, 95.270, 117.130 |
| Unit cell angles | 90.00, 131.56, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | a rough model of the selenomethionine-substituted protein |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 | 0.217 |
| Number of reflections | 47691 | |
| <I/σ(I)> | 24 | 8.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 25% PEG 6000, 0.1M MES, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
