2ZYG
Apo-form of dimeric 6-phosphogluconate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 112.748, 112.748, 118.939 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.1591 |
Rwork | 0.156 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zyd |
RMSD bond length | 0.162 |
RMSD bond angle | 1.634 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.139 | 0.520 |
Number of reflections | 51259 | |
<I/σ(I)> | 10.8 | 2.9 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.12M diammonium hydrogen citrate, 20% PEG3350, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |