2ZY5
R487A mutant of L-aspartate beta-decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.953, 216.215, 208.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.650 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zy4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.844 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.094 | 0.446 |
| Number of reflections | 97136 | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 92.2 | 94.8 |
| Redundancy | 1.69 | 1.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 15% PEG 4000, 0.1M lithium sulfate, 0.1M Tris-HCl (pH7.4), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
