2ZY3
dodecameric L-aspartate beta-decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.734, 216.141, 208.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zy2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.121 | 0.492 |
| Number of reflections | 115036 | |
| <I/σ(I)> | 11.2 | 2.4 |
| Completeness [%] | 98.6 | 96.1 |
| Redundancy | 4.3 | 4.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.4 | 277 | 0.1M Tris-HCl (pH 7.4), 0.1M Lithium sulfate, 15% PEG 4000, hanging drop, temperature 277K |
