2ZY3
dodecameric L-aspartate beta-decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL17B2 |
Synchrotron site | NSRRC |
Beamline | BL17B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-07-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 149.734, 216.141, 208.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zy2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.528 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.121 | 0.492 |
Number of reflections | 115036 | |
<I/σ(I)> | 11.2 | 2.4 |
Completeness [%] | 98.6 | 96.1 |
Redundancy | 4.3 | 4.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.4 | 277 | 0.1M Tris-HCl (pH 7.4), 0.1M Lithium sulfate, 15% PEG 4000, hanging drop, temperature 277K |