2ZXQ

Crystal structure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.96787, 0.97923, 0.97939, 0.96416
Spacegroup name	P 65
Unit cell lengths	192.267, 192.267, 123.017
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.490 - 2.000
R-factor	0.17324
Rwork	0.172
R-free	0.19564
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	172447
<I/σ(I)>	27.2	3.5
Completeness [%]	99.1	99.8
Redundancy	7.2	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	0.1M MES-NaOH, 3% PEG 20000, 25% MPD, 0.2M NaCl, 0.01M MnCl2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K