2ZXP

Crystal structure of RecJ in complex with Mn2+ from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2008-07-01
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000000, 1.700000
Spacegroup name	P 43 21 2
Unit cell lengths	83.127, 83.127, 249.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.560 - 2.300
Rwork	0.232
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENATRY 2ZXO
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.078	0.339
Number of reflections	40110
<I/σ(I)>	50.6	9
Completeness [%]	100.0	100
Redundancy	13.4	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.05M ADA, 6% PEG4000, 1% 2-propanol. The crystal was soaked in cryoprotecting solution containg 20% glycerol and 10mM manganese sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K