2ZXL
Crystal structure of red chlorophyll catabolite reductase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.286, 68.140, 83.273 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.22067 |
| Rwork | 0.216 |
| R-free | 0.29817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.293 |
| Number of reflections | 22833 | |
| <I/σ(I)> | 7.2 | 2.2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 25% PEG 4000, 0.1M ammonium sulfate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
