Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-12 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.634, 45.556, 78.043 |
| Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
| Resolution | 28.520 - 1.870 |
| R-factor | 0.18946 |
| Rwork | 0.187 |
| R-free | 0.23969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d1v |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.520 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.049 | 0.395 |
| Number of reflections | 18160 | |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 97.2 | 99.9 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25%(w/v) PEG3350, 0.2M Ammoniun acetate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
