2ZXH
Structure of Aquifex aeolicus GidA in the form I crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 101.638, 213.272, 231.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.200 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.126 | 0.398 |
| Number of reflections | 78176 | |
| <I/σ(I)> | 11.5 | 2.2 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 1.0M sodium/potassium phosphate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
