2ZXE

Crystal structure of the sodium - potassium pump in the E2.2K+.Pi state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	223.815, 50.914, 163.786
Unit cell angles	90.00, 105.12, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.24772
Rwork	0.246
R-free	0.27124
Starting model (for MR)	3b8e
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.058	0.372
Number of reflections	70074
<I/σ(I)>	38.8	2.8
Completeness [%]	99.1	95.7
Redundancy	17	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Dialysis	7	298	pH 7.0, Dialysis, temperature 298K
1	Dialysis	7	298	pH 7.0, Dialysis, temperature 298K