2ZXC
Ceramidase complexed with C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.741, 65.800, 340.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.970 - 2.200 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Native structure |
RMSD bond length | 0.010 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.144 | 0.420 |
Number of reflections | 103094 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 97.5 | 93.6 |
Redundancy | 4.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 14% PEG3350, 200mM Ammonium Formate, 1mM C2-seramide, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |