2ZX9
alpha-L-fucosidase complexed with inhibitor, B4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.808, 180.808, 169.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.450 - 2.620 |
| R-factor | 0.1654 |
| Rwork | 0.162 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 2zwz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.710 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.080 | 0.505 |
| Number of reflections | 32060 | |
| <I/σ(I)> | 19.1 | 2.8 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 17% PEG 5000 MME, 0.1M Tris-HCl, pH 7.6, 5% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
