2ZX6
alpha-L-fucosidase complexed with inhibitor, F10-1C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 179.811, 179.811, 168.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.270 - 2.420 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 2zwz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.068 | 0.504 |
| Number of reflections | 40040 | |
| <I/σ(I)> | 25.6 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 16% PEG 5000 MME, 0.1M Tris-HCl, pH 8.0, 5% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
