2ZX2
Rhamnose-binding lectin CSL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-07 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.690, 75.376, 94.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 1.800 |
| R-factor | 0.20825 |
| Rwork | 0.206 |
| R-free | 0.25623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zx0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 37718 | |
| <I/σ(I)> | 30.5 | 8.1 |
| Completeness [%] | 96.4 | 89.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 15% PEG8000, 50mM potassium phosphate, 20mM rhamnose, pH4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
