2ZX0
Rhamnose-binding lectin CSL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-07 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.040, 74.602, 94.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.19258 |
Rwork | 0.191 |
R-free | 0.22976 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 30454 | |
<I/σ(I)> | 13.1 | 1.9 |
Completeness [%] | 96.5 | 91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 15% PEG8000, 50mM potassium phosphate, pH4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 15% PEG8000, 50mM potassium phosphate, pH4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |