2ZWZ
alpha-L-fucosidase complexed with inhibitor, Core1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97315 |
Spacegroup name | H 3 2 |
Unit cell lengths | 180.693, 180.693, 169.375 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.360 |
R-factor | 0.162 |
Rwork | 0.159 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB enrty 1HL8 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.856 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.070 | 0.497 |
Number of reflections | 43077 | |
<I/σ(I)> | 21.4 | 2.9 |
Completeness [%] | 99.0 | 99.7 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 14% PEG 5000 MME, 0.1M HEPES, pH 7.4, 4% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |