2ZWZ
alpha-L-fucosidase complexed with inhibitor, Core1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.693, 180.693, 169.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.360 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB enrty 1HL8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.070 | 0.497 |
| Number of reflections | 43077 | |
| <I/σ(I)> | 21.4 | 2.9 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 14% PEG 5000 MME, 0.1M HEPES, pH 7.4, 4% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
