2ZWV

Crystal structure of Thermus thermophilus 16S rRNA methyltransferase RsmC (TTHA0533)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-03
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	50.178, 80.093, 93.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.720 - 2.000
R-factor	0.189
Rwork	0.189
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zul
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	26313
<I/σ(I)>	15	3.7
Completeness [%]	100.0	100
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	293	0.4M Potassium sodium tartrate, 0.25% Polyethylene glycol MME 5000, 0.05M TRIS pH8.5, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K