2ZWT
Crystal Structure of Ferric Cytochrome P450cam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.860, 63.860, 247.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.060 - 1.350 |
| R-factor | 0.16435 |
| Rwork | 0.163 |
| R-free | 0.18981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (9.4.09) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.060 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.064 | 0.400 |
| Number of reflections | 113101 | |
| <I/σ(I)> | 41.4 | 4.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 11-14% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
