2ZWN
Crystal structure of the novel two-domain type laccase from a metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-26 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.669, 100.945, 124.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.010 - 1.700 |
R-factor | 0.16054 |
Rwork | 0.159 |
R-free | 0.18322 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.010 |
High resolution limit [Å] | 1.700 |
Number of reflections | 98106 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Potassium chloride, 0.05M HEPES, pH7.5, 35% Pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, SITTING DROP, temperature 293K |