2ZWM
Crystal structure of YycF receiver domain from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 |
| Unit cell lengths | 58.824, 58.824, 78.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.470 - 2.040 |
| R-factor | 0.19543 |
| Rwork | 0.194 |
| R-free | 0.22982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a9r |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.886 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.470 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.062 | 0.417 |
| Number of reflections | 19194 | |
| <I/σ(I)> | 15.6 | 3.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2M Potassium sodium tartrate tetrahydrate, 0.1M tri-Sodium citrate dihydrate, 2.0M Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
