2ZWK
Crystal structure of intimin-Tir90 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2008-09-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 96.780, 96.780, 644.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.670 - 3.100 |
Rwork | 0.301 |
R-free | 0.36000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zqk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.670 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.125 | 0.413 |
Number of reflections | 26817 | |
<I/σ(I)> | 11.2 | 2.7 |
Completeness [%] | 93.4 | 95.3 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 2M (NH4)2SO4, 5% 2-Propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |