2ZWB
Neutron crystal structure of wild type human lysozyme in D2O
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | NUCLEAR REACTOR |
Source details | OTHER |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-01 |
Detector | JAERI BIX-3 |
Wavelength(s) | 2.88 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.825, 56.883, 60.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe0 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.211 | 0.491 |
Number of reflections | 25514 | |
<I/σ(I)> | 3.894 | 1.11 |
Completeness [%] | 69.0 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 4.3 | 293 | PH 4.30(PD), SMALL TUBES, temperature 293K |