2ZVZ
Structure of the periplasmic domain of MotB from Salmonella (crystal form III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 35 |
Detector technology | CCD |
Collection date | 2008-02-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.200, 46.240, 173.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.680 - 2.400 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zov |
RMSD bond length | 0.009 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.220 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 14884 | |
<I/σ(I)> | 17 | 5.4 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 22% PEG MME 2000, 0.1M Acetate, 0.1M MgSO4, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |