2ZVN
NEMO CoZi domain incomplex with diubiquitin in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.195, 141.288, 144.179 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.750 - 3.000 |
R-factor | 0.26336 |
Rwork | 0.261 |
R-free | 0.30274 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.097 | 0.057 | 0.604 |
Number of reflections | 24893 | ||
<I/σ(I)> | 19.934 | ||
Completeness [%] | 99.8 | 99.4 | 98.6 |
Redundancy | 7.1 | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |