2ZVI
Crystal structure of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.334, 91.466, 106.967 |
| Unit cell angles | 90.00, 90.82, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oek |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.273 |
| Number of reflections | 64719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.14M Na Thiocyanate, 21% (w/v) polyethylene glycol 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
