2ZVD
Crystal structure of Pseudomonas sp. MIS38 lipase in an open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-22 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 104.781, 104.781, 497.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.150 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20979 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z8x |
RMSD bond length | 0.017 |
RMSD bond angle | 1.585 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.103 | 0.406 |
Number of reflections | 95813 | |
<I/σ(I)> | 27.7 | 6.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M ammonium acetate, 0.1M Tris-HCl, pH8.5, 30% isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |