2ZUW
Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-03-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 67.905, 112.039, 118.662 |
Unit cell angles | 105.21, 90.43, 107.32 |
Refinement procedure
Resolution | 41.070 - 2.110 |
R-factor | 0.17184 |
Rwork | 0.169 |
R-free | 0.22479 |
Structure solution method | MAD |
RMSD bond length | 0.036 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.089 | 0.327 |
Number of reflections | 180455 | |
<I/σ(I)> | 15.936 | 2.8 |
Completeness [%] | 98.2 | 97.1 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, MgSO4, pH 6.5, VAPOR DIFFUSION, temperature 277K | |
1 | 6.5 | 277 | Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, MgSO4, pH 6.5, VAPOR DIFFUSION, temperature 277K |