2ZUT
Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GalNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-03-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 67.861, 111.658, 118.660 |
Unit cell angles | 105.20, 90.48, 107.27 |
Refinement procedure
Resolution | 44.810 - 1.900 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.368 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | 0.232 |
Number of reflections | 243435 | |
<I/σ(I)> | 21.066 | 3.5 |
Completeness [%] | 97.0 | 96 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | Na cacodyrate, Mg(NO3)2, PEG 4000, GalNAc, pH 6.5, VAPOR DIFFUSION, temperature 277K |
1 | VAPOR DIFFUSION | 6.5 | 277 | Na cacodyrate, Mg(NO3)2, PEG 4000, GalNAc, pH 6.5, VAPOR DIFFUSION, temperature 277K |