2ZUJ
Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant(D297L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-02 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.758, 63.758, 248.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.390 - 1.600 |
| R-factor | 0.16251 |
| Rwork | 0.160 |
| R-free | 0.20275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zax |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (9.4.09) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.390 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.398 |
| Total number of observations | 307596 | |
| Number of reflections | 66343 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 96.5 | 98.9 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 11-14% PEG 8000, 250mM potassium chloride, 10mM DTE, 1mM d-camphor, 50mM Tris-HCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
