2ZUI
Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.604, 63.604, 250.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.450 - 1.500 |
| R-factor | 0.15921 |
| Rwork | 0.158 |
| R-free | 0.17808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zax |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.467 |
| Data reduction software | DPS |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (9.4.09) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.450 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.185 | |
| Total number of observations | 557635 | |
| Number of reflections | 80010 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 95.7 | 72.1 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 12-15% PEG 4000, 50mM Tris-HCl, 250mM KCl, 1mM d-Camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
