2ZUH
Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-02 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.749, 63.749, 250.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.540 - 1.550 |
R-factor | 0.1617 |
Rwork | 0.160 |
R-free | 0.18471 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zax |
RMSD bond length | 0.011 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (9.4.09) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.540 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 75680 | |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 7.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 12-15% PEG 4000, 50mM Tris-HCl, 250mM KCl, 0.1mM d-Camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |