2ZUA
Crystal structure of nucleoside diphosphate kinase from Haloarcula quadrata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 73.571, 73.571, 214.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.570 - 2.590 |
| R-factor | 0.22524 |
| Rwork | 0.222 |
| R-free | 0.28940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bhn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.590 |
| Rmerge | 0.084 |
| Number of reflections | 20457 |
| <I/σ(I)> | 30.7 |
| Completeness [%] | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 100mM HEPES-NaOH, 25% PEG400, 10mM EDTA, pH7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
