2ZTT
Crystal Structure of RNA polymerase PB1-PB2 subunits from Influenza A Virus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97898,0.97931,0.9832 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.273, 61.477, 45.473 |
| Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.23452 |
| Rwork | 0.232 |
| R-free | 0.27164 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.131 |
| Number of reflections | 13052 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 92.9 | 85.6 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 0.1M potassium phosphate, 15% PEG 4000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
