2ZTT
Crystal Structure of RNA polymerase PB1-PB2 subunits from Influenza A Virus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2008-06-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97898,0.97931,0.9832 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.273, 61.477, 45.473 |
Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.23452 |
Rwork | 0.232 |
R-free | 0.27164 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 2.066 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.049 | 0.131 |
Number of reflections | 13052 | |
<I/σ(I)> | 20.5 | |
Completeness [%] | 92.9 | 85.6 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 0.1M potassium phosphate, 15% PEG 4000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |