2ZTS
Crystal structure of KaiC-like protein PH0186 from hyperthermophilic archaea Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97905, 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.655, 51.807, 97.468 |
| Unit cell angles | 90.00, 122.83, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.070 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.09) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.083 | 0.050 | 0.509 |
| Number of reflections | 46906 | ||
| <I/σ(I)> | 26.462 | ||
| Completeness [%] | 95.5 | 99.8 | 73.4 |
| Redundancy | 6.8 | 7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 9% ethanol, 100mM imidazole pH7.6, 200mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
