2ZSO
Carbonmonoxy Sperm Whale Myoglobin at 100 K: Laser on [450 min]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW14A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW14A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.827 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.342, 30.635, 63.714 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.210 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mbc |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.208 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.250 |
| High resolution limit [Å] | 1.210 | 2.610 | 1.210 |
| Rmerge | 0.052 | 0.029 | 0.408 |
| Number of reflections | 34942 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 88.4 | 94.8 | 36.6 |
| Redundancy | 3.5 | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 6 | 293 | 2.4M Ammonium sulfate, 10mM TrisHCl, 100mM Sodium phosphate, pH 6.0, batch, temperature 293K |
