2ZSJ
Crystal structure of threonine synthase from Aquifex aeolicus VF5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.085, 71.857, 99.456 |
| Unit cell angles | 75.44, 75.30, 89.99 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uim |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.083 | 0.350 |
| Number of reflections | 129161 | |
| <I/σ(I)> | 6.7 | 1.59 |
| Completeness [%] | 93.2 | 82.5 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 295 | 0.2M ammonium acetate, 25%(w/v) PEG3350, 0.1M bis-tris, pH6.1, microbatch, temperature 295K |
