2ZSH
Structural basis of gibberellin(GA3)-induced DELLA recognition by the gibberellin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.99533 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.018, 82.018, 130.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.205 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.129 |
Number of reflections | 42175 | |
Completeness [%] | 100.0 | 98.4 |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl, 0.8M LiCl2, 26% PEG 4000, 1mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |