2ZQP
Crystal Structure of SecYE translocon from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 91.876, 91.876, 240.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.920 - 6.000 |
R-factor | 0.40884 |
Rwork | 0.407 |
R-free | 0.44504 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the SecYE structure produced by MD simulation using 2ZJS structure |
RMSD bond length | 0.011 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 6.000 |
Number of reflections | 2743 |
Completeness [%] | 93.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 28.5% PEG 3350, 0.18M Na-citrate, 0.015% octaethylene glycol monododecyl ether, 5% glycerol, 0.0001M Pefabloc, VAPOR DIFFUSION, SITTING DROP, temperature 293K |