2ZQM
Crystal structure of the prefoldin beta subunit from Thermococcus strain KS-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-12-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 70.603, 70.603, 114.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19400 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 11352 | |
| <I/σ(I)> | 43.2 | 6.3 |
| Completeness [%] | 96.4 | 93.5 |
| Redundancy | 9.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 291 | 25% (v/v) 1,2-propanediol, phosphate-citrate pH 4.2, 5% (v/v) PEG 3000, 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
