2ZQE
Crystal structure of the Smr domain of Thermus thermophilus MutS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-11 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.813, 54.216, 30.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.900 - 1.700 |
| R-factor | 0.19074 |
| Rwork | 0.189 |
| R-free | 0.22699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d9i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 | 0.137 |
| Number of reflections | 9284 | |
| <I/σ(I)> | 36.4 | 13.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1M Citric acid, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
