2ZPO
Crystal Structure of Green Turtle Egg White Ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-12 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.271, 34.174, 39.738 |
| Unit cell angles | 90.00, 102.03, 90.00 |
Refinement procedure
| Resolution | 21.720 - 1.600 |
| R-factor | 0.18608 |
| Rwork | 0.183 |
| R-free | 0.24722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwl |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.182 |
| High resolution limit [Å] | 1.599 |
| Number of reflections | 14850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.1M Ammonium sulfate, 30% glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
