2ZP7
Crystal structure of LysN, alpha-aminoadipate aminotransferase (Leucine complex), from Thermus thermophilus HB27
Replaces: 2ZG5Replaces: 2DTVExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.948, 167.639, 119.358 |
Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
Resolution | 41.920 - 2.260 |
R-factor | 0.20753 |
Rwork | 0.205 |
R-free | 0.26292 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.499 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.122 | 0.295 |
Number of reflections | 101430 | |
<I/σ(I)> | 19.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |