2ZOV

Structure of the periplasmic domain of MotB from Salmonella (crystal form I)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979101,0.979397,0.9730,0.9860
Spacegroup name	P 32 2 1
Unit cell lengths	99.740, 99.740, 43.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.650 - 2.000
R-factor	0.233
Rwork	0.233
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	17162
<I/σ(I)>	22.1	8
Completeness [%]	99.5	99.4
Redundancy	9.7	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	6% PEG 4000, 0.1M Acetate, 0.22M Zn(OAc)2, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K