2ZOV
Structure of the periplasmic domain of MotB from Salmonella (crystal form I)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979101,0.979397,0.9730,0.9860 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.740, 99.740, 43.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.650 - 2.000 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.26500 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 17162 | |
<I/σ(I)> | 22.1 | 8 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 9.7 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 6% PEG 4000, 0.1M Acetate, 0.22M Zn(OAc)2, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |