2ZOT

Crystal structure of human F-spondin reeler domain (fragment 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	90
Detector technology	CCD
Collection date	2006-12-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 43
Unit cell lengths	57.579, 57.579, 190.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.540 - 2.700
R-factor	0.24563
Rwork	0.242
R-free	0.31251
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	16670
<I/σ(I)>	9	2.038
Completeness [%]	100.0	99.7
Redundancy	4.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M bis-tris(pH 5.5), 0.4M ammonium acetate, 25% polyethylene glycol(PEG) 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M bis-tris(pH 5.5), 0.4M ammonium acetate, 25% polyethylene glycol(PEG) 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M bis-tris(pH 5.5), 0.4M ammonium acetate, 25% polyethylene glycol(PEG) 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K